Journal: PLoS ONE

Article Title: A Structural Domain Mediates Attachment of Ethanolamine Phosphoglycerol to Eukaryotic Elongation Factor 1A in Trypanosoma brucei

doi: 10.1371/journal.pone.0009486

Figure Lengend Snippet: (A), (B), T. brucei Δprocyclin#1 expressing HA-Alba-III and PTP-III fusion proteins were labeled with [ 3 H]Etn for 18 h. Proteins in the cell lysates (L), supernatants (SN), wash solutions (W) and immunoprecipitates (IP) were analyzed by SDS-PAGE using 12% acrylamide gels. SDS PAGE was followed either by immunoblotting (upper panels) or fluorography (lower panels). For immunoblotting α-HA antibody or α-IgG were used to detect HA-Alba-III or PTP-III, respectively. The lanes contain material from 1×10 7 parasites, except for the lanes with the immunoprecipitates in the fluorographs, which contain material prepared from 1.8×10 8 cell equivalents.

Article Snippet: The supernatant was supplemented with either anti-HA Affinity Matrix (Roche Diagnostics) or IgG Sepharose 6 Fast Flow matrix (GE Healthcare, Otelfingen, Switzerland) and incubated on a rotating device overnight at 4°C.

Techniques: Expressing, Labeling, SDS Page, Western Blot

Journal: Cell

Article Title: Heteromeric RNP Assembly at LINEs Controls Lineage-Specific RNA Processing

doi: 10.1016/j.cell.2018.07.001

Figure Lengend Snippet:

Article Snippet: Blue Sepharose 6 Fast Flow/ HisTrap HP columns , GE LifeSciences , Cat# 17-0412-01.

Techniques: Recombinant, Sample Prep, Sequencing, Binding Assay, Clone Assay, Software